C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12N 9/12 (2006.01) C12N 15/52 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2357540
The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.
L'invention décrit l'expression hétérologue de la transcriptase inverse du virus de la myéloblastose aviaire (AMV-RT) dans des cellules procaryotes et dans des souches particulières d'Escherichia coli (E. coli). L'invention comprend également certaines mesures destinées à simplifier la purification de l'AMV-RT hétérodimérique.
Frey Bruno
Mueller Rainer
Pajatsch Markus
Pallua Peter
Schmidt Manfred
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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