C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2465377
Methods for producing and identifying fragments of proteins, and more particularly to methods for generating and identifying soluble protein domains are disclosed based on a method for generating a library of nucleic acid fragments from nucleic acid encoding a desired polypeptide, and more especially a library of essentially, randomly sampled fragments of coding DNA sequence predominantly of defined size range and a method for selecting cloned gene fragments from the library that encode soluble protein domains.
L'invention concerne des procédés de production et d'identification de fragments de protéines, et plus particulièrement des procédés de production et d'identification de domaines protéiques solubles. L'invention repose notamment sur deux procédés : un procédé pour produire une banque de fragments d'acide nucléique à partir d'acide nucléique codant un polypeptide voulu, en particulier une banque comprenant essentiellement des fragments, prélevés de façon aléatoire, d'une séquence d'ADN codante, principalement d'une gamme de tailles définie, et un procédé pour sélectionner dans cette banque des fragments de gènes clonés codant des domaines protéiques solubles.
Driscoll Paul
Mcalister Mark
Pearl Laurence
Prodromou Chrisostomos
Savva Renos
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Birkbeck College
Domainex Limited
The Institute Of Cancer Research: Royal Cancer Hospital
University College London
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