Method for producing antibodies acting against a polypeptide...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/00 (2006.01) A61K 48/00 (2006.01) C07K 16/42 (2006.01)

Patent

CA 2350078

The present invention relates to a method for producing antibodies reacting specifically with a polypeptide that only recognises the coding nucleic acid, wherein said method comprises the following steps: a) the DNA coding the polypeptide is expressed in a host cell using a vector having at least one sequence coding a detection signal, and the expressed polypeptide is bound to a solid phase using the detection signal; b) regardless of step a), the DNA coding the polypeptide is introduced directly into an animal, which causes the expression of the polypeptide in the animal thus inducing the formation of antibodies acting against the polypeptide; and c), the polypeptide produced in step a) is used for reacting the antibodies formed in step b), for detecting them or for enriching them.

L'invention concerne un procédé permettant de préparer des anticorps qui réagissent de manière spécifique avec un polypeptide qui connaît l'acide nucléique codant. A) l'ADN qui code le polypeptide est exprimé dans une cellule hôte à l'aide d'un vecteur présentant au moins une séquence codant un signal de détection, et le polypeptide exprimé est lié à une phase solide à l'aide du signal de détection; b) indépendamment de l'étape a), l'ADN codant le polypeptide est introduit directement dans un animal, cette opération entraînant l'expression du polypeptide chez l'animal, qui induit la formation d'anticorps agissant à l'encontre du polypeptide et c) le polypeptide formé à l'étape a) est utilisé pour faire réagir les anticorps formés à l'étape b), les mettre en évidence ou les enrichir.

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