C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) C07K 14/415 (2006.01) C11B 1/02 (2006.01) C12N 9/02 (2006.01)
Patent
CA 2617714
The invention relates to a method for producing arachidonic acid and/or eicosapentaenoic acid in useful transgenic plants, according to which nucleic acids, which encode polypeptides with .DELTA.-6-desaturase activity, .DELTA.-6-elongase activity or .DELTA.-5-desaturase activity, are introduced into the plants. As an added advantage, a gene that encodes a .DELTA.-3-desaturase is expressed in the useful plants. In an additional embodiment of the method, additional nucleic acid sequences, which encode polypeptides involved in the biosynthesis of the fatty acid or lipid metabolism, can be expressed in the plants. Particularly advantageous nucleic acid sequences are those that encode .DELTA.-8-desaturase activity, .DELTA.-12-desaturase activity, .DELTA.-15-desaturase activity, .DELTA.-4-desaturase activity, .DELTA.-9-elongase activity and/or .DELTA.-5-elongase activity. The invention also relates to the use of the oils, lipids and/or fatty acids that have been produced by said method in animal feed or foodstuffs, cosmetics or pharmaceutical products.
L'invention concerne un procédé permettant de produire de l'acide arachidonique et/ou de l'acide eicosapentanoïque dans des plantes utiles transgéniques, selon lequel des acides nucléiques sont introduits dans la plante concernée, qui codent des polypeptides à activité .DELTA.-6-désaturase, .DELTA.-6-élongase ou .DELTA.-5-désaturase. Avantageusement, un gène codant une .omega.-3-désaturase est exprimé dans les plantes utiles. Dans un autre mode de réalisation avantageux du procédé, d'autres séquences d'acide nucléique codant des polypeptides de la biosynthèse du métabolisme d'acides gras ou du métabolisme lipidique sont exprimées dans les plantes. A cet effet, les séquences d'acide nucléique qui codent des polypeptides à activité .DELTA.-8-désaturase, .DELTA.-12-désaturease, .DELTA.-15-désaturase, .DELTA.-4-désaturase, .DELTA.-9-élongase et/ou .DELTA.-5-élongase sont particulièrement avantageuses. L'invention concerne par ailleurs l'utilisation des huiles, lipides et/ou acides gras obtenus au moyen dudit procédé, dans des aliments pour animaux ou des produits alimentaires, des cosmétiques ou des produits pharmaceutiques.
Bauer Joerg
Cirpus Petra
Datla Nagamani
Qiu Xiao
Truksa Martin
Basf Plant Science Gmbh
Robic
LandOfFree
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