Method for producing arachidonic acid in transgenic organisms

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C12N 1/19 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/63 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2455163

The invention relates to a method for the production of arachidonic acid in transgenic organisms, especially in transgenic plants and yeasts. The invention also relates to DNA sequences coding for a protein with enzymatic activity of a .DELTA.5-desaturase from Phytophthera megasperma. The invention further relates to transgenic plants and plant cell and transgenic yeasts containing a nucleic acid molecule comprising a DNA sequence according to the present invention and having, on the basis thereof, an enhanced arachidonic acid synthesis in comparison with wild-type cells. The invention also relates to harvest products and propagating material of transgenic plants.

L'invention se rapporte à un procédé de production d'acide arachidonique dans des organismes transgéniques, en particulier dans des plantes et des levures transgéniques. L'invention concerne également des séquences d'ADN codant pour une protéine présentant l'activité enzymatique d'une ?-5 désaturase issue de phytophthera megasperma. L'invention se rapporte en outre à des plantes et cellules végétales transgéniques ainsi qu'à des levures transgéniques lesquelles contiennent une molécule d'acide nucléique comportant une séquence d'ADN selon la présente invention et dont la synthèse d'acide arachidonique est de ce fait accrue comparativement à celle de cellules de type sauvage. L'invention concerne par ailleurs des produits de récolte et des substances de multiplication des plantes transgéniques.

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