Method for producing artificial skin

Details Method for producing artificial skin Method for producing artificial skin

A - Human Necessities

61

L

A61L 27/00 (2006.01)

Patent

CA 2716752

An object of the invention is to provide artificial skin that does not contain any animal-derived material or pathogen and has excellent biocompatibility. The invention provides, as a solution means, a method for producing artificial skin comprising the steps of: (A) forming a dermal layer by solidifying a mixture of dermal fibroblasts and a peptide hydrogel having a fibrous structure; and (B) forming an epidermal layer by seeding skin keratinocytes onto the dermal layer obtained in Step (A), and culturing the epidermal keratinocytes.

L'invention concerne une peau artificielle qui ne contient pas de matière d'origine animale ou un pathogène et a une excellente biocompatibilité. Comme moyen pour la résolution de ce problème, l'invention porte sur un procédé de fabrication d'une peau artificielle consistant à : (A) former une couche dermique par solidification d'un mélange de fibroblastes dermiques et d'un hydrogel peptidique ayant une structure fibreuse; et (B) former une couche épidermique par ensemencement et culture de kératinocytes de peau sur la couche dermique obtenue dans l'étape (A).

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