Method for producing baccatin

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/10 (2006.01) C07D 305/14 (2006.01) C07K 1/14 (2006.01) C12P 17/02 (2006.01)

Patent

CA 2319136

A process for preparing baccatin and/or baccatin derivatives is described in which 10-deacetylbaccatin or a 10-deacetylbaccatin derivative is reacted in the presence of an isolated enzyme and an acetyl donor, the enzyme used being an acetyl transferase having a molecular weight of from 70 to 72 kD, determined by SDS-PAGE, which acetyl transferase is obtainable from Taxus chinensis cell cultures.

L'invention concerne un procédé de préparation de baccatine et/ou de dérivés de baccatine, selon lequel on fait réagir de la 10-désacétylbaccatine ou un dérivé de 10-désacétylbaccatine en présence d'un enzyme isolé et d'un donneur acétyle. L'enzyme utilisé est une acétyltransférase avec un poids moléculaire allant de 70 à 72 kD, déterminé par SDS-PAGE, obtenue à partir de cultures cellulaires de Taxus chinensis.

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