Method for producing basic amino acid

Details Method for producing basic amino acid Method for producing basic amino acid

C - Chemistry, Metallurgy

12

P

C12P 13/04 (2006.01) C12P 13/08 (2006.01) C12P 13/10 (2006.01) C12P 13/24 (2006.01)

Patent

CA 2355492

When a basic amino acid is produced by culturing a microorganism having an ability to produce the basic amino acid in a liquid medium under an aerobic condition to produce and accumulate the basic amino acid in the medium, pH of the medium is controlled to be 6.5-9.0 during the culture, and 7.2-9.0 at the end of the culture, and a culture period where 2 g/L or more of hydrogencarbonate ions and/or carbonate ions exist in the medium is secured during the culture by controlling pressure in a fermentation tank to be a positive pressure during the fermentation, or supplying carbon dioxide gas or a mixed gas containing carbon dioxide gas to the medium, so that the hydrogencarbonate ions and/or carbonate ions should serve as counter ions of cations mainly consisting of the basic amino acid.

Lorsqu'un acide aminé basique est produit par culture d'un microorganisme ayant une aptitude à produire l'acide aminé basique dans un milieu liquide dans des conditions aérobies, de manière à produire et accumuler l'acide aminé basique dans le milieu, le pH du milieu est commandé pour être 6,5 -9,0 lors de la culture et 7,2 -9,0 à la fin de la culture, et on assure une période de culture où 2 g/L ou plus d'ions d'hydrogénocarbonate et/ou des ions de carbonate existent dans le milieu durant la culture en contrôlant la pression dans une cuve de fermentation pour qu'elle soit à pression positive pendant la fermentation, ou en acheminant du dioxyde de carbone ou d'un gaz mixte contenant du dioxyde de carbone au milieu, de sorte que des ions d'hydrogénocarbonate et/ou des ions de carbonate servent de contre-ions de cations constitués principalement de l'acide aminé basique.

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