C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/35
C12N 11/08 (2006.01)
Patent
CA 1143680
ABSTRACT OF THE DISCLOSURE The invention refers to a process for the manufacture of mechanically and chemically stable, porous biocatalysts with high enzymatic activity by including whole cells, cell fragments, or enzymes in polymers, whereby mixture (A) is in- serted into mixture (B) as a precipitation bath. Mixture (A) is composed of an enzymatically active substance in an aqueous solution or suspension and a water- soluble polyelectrolyte, or of an enzymatically active substance in an aqueous solution or suspension of an epoxyprepolymer component, a multifunctional harden- ing component and a water-soluble polyelectrolyte, and mixture (B) is composed of the aqueous solution of a compound containing polyvalent ions charged oppositely to the polyelectrolytes. When stirred, the bead-like particles that have formed are solidified, washed, and following filtering off are carefully dried to contraction and solidification, and hardened, washed and discharged in the moist condition in mixture (B), or washed and discharged in the moist condition follow- ing solidification of the epoxy matrix in a phosphate buffer solution for dis- solving out the polyelectrolytes.
346851
Eng Harald
Klein Joachim
Vorlop Klaus-Dieter
Wagner Fritz
Eng Harald
Fetherstonhaugh & Co.
Klein Joachim
Vorlop Klaus-Dieter
Wagner Fritz
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