C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/00 (2006.01) A61K 39/12 (2006.01) A61K 39/145 (2006.01) A61K 39/15 (2006.01) A61K 39/245 (2006.01) C07K 14/11 (2006.01) C12N 7/02 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2205015
The present invention includes an approach for producing viruses, such as Influenza, and vaccines derived therefrom as well as recombinant proteins derived from viral vectors, by utilizing vertebrate cells cultured under protein-free conditions. These cells, which include a cellular biomass, show improved capabilities for propagating viruses, and eliminate the need for costly and time-consuming viral passaging and purification. The invention also includes further approaches for enhancing the propagation of virus by employing activating substances, modifying the activation site of viruses and using augmentation loops. Improved approaches for producing viral reassortants also are provided.
L'invention décrit une approche de production de virus, tels que celui de la grippe, et de vaccins dérivés de ceux-ci, de même que de protéines recombinées dérivées de vecteurs viraux, à l'aide de cellules de vertébrés mises en culture dans un milieu dépourvu de protéines. Ces cellules qui comprennent une biomasse cellulaire présentent de bonnes capacités de propagation de virus, et leur production ne nécessite ni passage viral ni purification, coûteux en temps et en argent. L'invention décrit en outre des approches destinées à augmenter la propagation de virus par l'emploi de substances activantes, par la modification du site d'activation de virus et l'utilisation de boucles favorisant cette augmentation. On décrit également des approches perfectionnées de production d'agents de réassortiment phénotypiques viraux.
Barrett Noel
Dorner Friedrich
Kistner Otfried
Mundt Wolfgang
Baxter Healthcare S.a.
Immuno Aktiengesellschaft
Smart & Biggar
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