Method for producing biologicals in protein-free culture

Details Method for producing biologicals in protein-free culture Method for producing biologicals in protein-free culture

C - Chemistry, Metallurgy

12

N

C12N 7/00 (2006.01) A61K 39/12 (2006.01) A61K 39/145 (2006.01) C07K 14/11 (2006.01)

Patent

CA 2415990

The present invention includes an approach for producing viruses, such as Influenza, and vaccines derived therefrom as well as recombinant proteins derived from viral vectors, by utilizing vertebrate cells cultured under protein-free conditions. These cells, which include a cellular biomass, show improved capabilities for propagating viruses, and eliminate the need for costly and time-consuming viral passaging and purification. The invention also includes further approaches for enhancing the propagation of virus by employing activating substances, modifying the activation site of viruses and using augmentation loops. Improved approaches for producing viral reassortants also are provided.

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