Method for producing carbohydrates

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 5/06 (2006.01) A61K 31/70 (2006.01) C07H 13/04 (2006.01) C12N 9/64 (2006.01) C12P 19/26 (2006.01)

Patent

CA 2091521

X-8833 Abstract The present invention relates to a method for producing peptides containing oligosaccharides with the sequence GalNAc.beta.(1-4)(Fuc.alpha.(1-3)GlcNAc by incubatiny Human Kidney 293 cells expressing a peptide capable of being glycosylated under conditions suitable for expression of the peptide, then recovering the peptide from the cell or cell culture supernatant. The invention also comprises methods for further isolating the novel oligosaccharides from the peptide produced in 293 cells. The peptides and/or oligosaccharides produced can be used to act as high affinity ligands of certain cell adhesion receptors. The peptides and/or oligosaccharides may be used to prevent or reduce cell adhesion and inflammation.

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