Method for producing chiral carboxylic acids from nitriles...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/78 (2006.01) C07H 21/00 (2006.01) C12N 1/21 (2006.01) C12N 15/55 (2006.01) C12N 15/63 (2006.01) C12P 7/42 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2347521

The invention relates to nucleic acid sequences which code for a polypeptide with nitrilase activity, to nucleic acid constructs containing the nucleic acid sequences, and to vectors containing the nucleic acid sequences or the nucleic acid constructs. The invention also relates to amino acid sequences which are coded by the nucleic acid sequences, and to microorganisms containing the nucleic acid sequences, the nucleic acid constructs or vectors containing the nucleic acid sequences or the nucleic acid constructs. In addition, the invention relates to a method for producing chiral carboxylic acids from the racemic nitriles.

L'invention concerne des séquences nucléotidiques qui codent pour un polypeptide présentant une activité de nitrilase, des acides nucléiques de recombinaison contenant lesdites séquences nucléotidiques, ainsi que des vecteurs contenant lesdites séquences nucléotidiques ou les acides nucléiques de recombinaison. L'invention concerne en outre des séquences d'aminoacides qui sont codées par lesdites séquences nucléotidiques et des micro-organismes contenant lesdites séquences nucléotidiques, les acides nucléiques de recombinaison ou des vecteurs contenant les séquences nucléotidiques ou les acides nucléiques de recombinaison. De plus, l'invention concerne un procédé de production d'acides carboxyliques chiraux à partir des nitriles racémiques.

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