Method for producing dna

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01) C12N 15/64 (2006.01) C12N 15/66 (2006.01)

Patent

CA 2408977

A vector comprising one recognition sequence of a first restriction enzyme of which digestion site exists at a particular position with respect to the recognition sequence and does not exist within the recognition sequence and one recognition sequence of a second restriction enzyme of which digestion site is specific, wherein a fragment obtained by digestion of the vector with the first restriction enzyme can be digested with the second restriction enzyme and a distance between the digestion site of the first restriction enzyme and the digestion site of the second restriction enzyme is 30 nucleotides or shorter, and a method for producing DNA having a target sequence by using the vector.

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