C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) A01H 5/00 (2006.01) C11B 1/00 (2006.01) C11C 1/04 (2006.01) C11C 3/00 (2006.01) C12N 9/20 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)
Patent
CA 2195560
A method for producing fatty acids or derivatives thereof from oil plants by producing transgenic oil plants that have at least one gene coding for a lipase, i.e. a lipase gene, and a promoter combined with the lipase gene and enabling the expression thereof either in compartments other than the lipid accumulation compartments or by exogenous induction, collecting seeds or fruits containing lipids of the plants, crushing them, optionally after inducer processing, to contact the lipids with the lipase, incubating the resulting material to cause enzymatic hydrolysis of the lipids, and extracting the fatty acids or fatty acid derivatives.
L'invention concerne un procédé de production d'acides gras ou de dérivés d'acides gras à partir de plantes oléagineuses. Ce procédé est caractérisé en ce qu'on produit des plantes oléagineuses transgéniques possédant, d'une part, au moins un gène codant pour une enzyme lipase, dit gène de lipase, d'autre part, associé à ce gène de lipase, un promoteur permettant une expression dudit gène soit dans des compartiments différents des compartiments d'accumulation des lipides, soit sur induction exogène, on recueille les graines ou fruits contenant les lipides des plantes, on les broie le cas échéant après traitement inducteur de façon à mettre en contact lipides et lipase, on laisse incuber l'ensemble pour engendrer une hydrolyse enzymatique des lipides et on extrait les acides gras ou dérivés.
Alibert Gilbert
Boudet Alain
Mouloungui Zephirin
Institut National Polytechnique de Toulouse (i.n.p.t.)
Mcfadden Fincham
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