Method for producing hepatitis b virus proteins in yeast

Details Method for producing hepatitis b virus proteins in yeast Method for producing hepatitis b virus proteins in yeast

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.3

C12N 15/51 (2006.01) C07K 14/02 (2006.01) C12N 1/16 (2006.01) C12N 15/81 (2006.01) G01N 33/576 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1334386

The hepatitis B virus preS2 antigen gene linked in one contiguous reading frame to the hepatitis B virus surface antigen gene has been expressed in Saccharomyces cerevisiae utilizing an optimized plasmid construction. The expressed protein aggregates into a particulate form which displays the major antigenic sites encoded by both domains, thereby highlighting the utility of yeast as a host for the high level expression of the preS2 as well as the S domain. This protein is useful in in vitro diagnostic systems and as a vaccine for the treatment and prevention of hepatitis B virus-induced diseases and/or infections.

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