Method for producing heterologous proteins in a homothallic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/80 (2006.01) C12N 1/15 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2433430

The invention relates to a method for producing a heterologous protein in a filamentous fungus and to suitable promoters therefor. The invention also relates to vectors and host cells and to a kit and the use thereof. The aim of the invention is to provide a method for producing a heterologous protein in a filamentous fungus, which allows the efficient production of heterologous proteins and in which a contamination of the created protein by vegetative spores is prevented. To achieve this, the invention discloses a method for producing a heterologous protein in a filamentous fungus, which comprises (a) the cultivation of a homothallic fungus of the Sordariaceae family, which contains an expression cassette with the following elements in a functional combination: a promoter that is active in the fungus of the Sordariaceae family; a heterologous gene and a terminator that is active in the fungus of the Sordariaceae family and (b) the harvesting of the created protein in a manner known per se.

La présente invention concerne un procédé de fabrication d'une protéine hétérologue dans un champignon filamenteux, ainsi que des promoteurs destinés à cet effet. L'invention concerne également des vecteurs et des cellules hôtes, ainsi qu'un ensemble et l'utilisation de ceux-ci. L'invention vise à mettre en oeuvre un procédé de fabrication d'une protéine hétérologue dans un champignon filamenteux permettant une production efficace de protéines hétérologues, et d'empêcher toute contamination de la protéine produite par des spores végétatives. A cet effet, ledit procédé de fabrication d'une protéine hétérologue dans un champignon filamenteux consiste (a) à cultiver un champignon homothallique de la famille Sordariaceae comportant une cassette d'expression, ladite cassette contenant en combinaison fonctionnelle les éléments suivants : un promoteur actif dans le champignon de la famille Sordariaceae, un gène hétérologue et un terminateur actif dans le champignon de la famille Sordariaceae ; et, (b) à récupérer la protéine produite de manière connue en soi.

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