C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/877 (2010.01) C12N 5/073 (2010.01) C12N 15/87 (2006.01)
Patent
CA 2334953
The present invention provides a method for producing human cloned embryos by employing inter-species nuclear transplantation technique. The method for producing human cloned embryos of the invention comprises the steps of: preparing donor somatic cell lines collected from human; maturing oocytes collected from ovary of cow in vitro; removing the cumulus cells surrounding the oocytes; cutting a portion of zona pellucida of the matured oocytes to make a slit, and squeezing out a portion of cytoplasm including the first polar body through the slit to give enucleated recipient oocytes; transferring a nucleus to the recipient oocyte by injection of the donor cells to the enucleated recipient oocytes, followed by the subsequent electrofusion and activation of the electrofused cells to give embryos; and, postactivating and culturing the embryos in vitro. The human cloned embryos of the invention can be employed to obtain the human embryonic stem cells, which may be widely applied in biological and medical fields.
L'invention porte sur un procédé d'obtention d'embryons humains clonés au moyen de techniques de transplantation inter-espèces de noyaux. Ledit procédé comporte les étapes suivantes: préparation de souches de cellules somatiques donneuses de provenance humaine; maturation <i>in vitro</i> d'oocytes d'ovaires de vaches; élimination des cellules cumulus entourant les oocytes; incision d'une partie de la zona pellucida des oocytes mûrs; expression par la fente d'une partie du cytoplasme comportant le premier corps polaire de manière à obtenir des oocytes récepteurs énucléés; transfert par injection dans les oocytes récepteurs énucléés d'un noyau de cellule donneuse; électrofusion puis activation des cellules de manière à obtenir des embryons; postactivation puis mise en culture <i>in vitro</i> des embryons. Les embryons humains clonés de l'invention peuvent servir à l'obtention de cellules souches d'embryons humains aux nombreuses applications biologiques et médicales.
Cho Jong-Ki
Choi Yun-Hee
Han Jae-Yong
Hwang Woo-Suk
Kim Ki-Yon
Cassan Maclean
Hwang Woo-Suk
LandOfFree
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