Method for producing human leukocyte interferon .alpha.-2

Details Method for producing human leukocyte interferon .alpha.-2 Method for producing human leukocyte interferon .alpha.-2

C - Chemistry, Metallurgy

12

P

167/103.1

C12P 21/00 (2006.01) C07K 14/56 (2006.01) C12N 15/78 (2006.01)

Patent

CA 1264294

Abstract of the Disclosure A method for producing human leukocyte interferon alpha-2 resides in that there is carried out submerged cultivation of a producer strain Pseudo- monas species VG-84 carrying a plasmid pVG3 with an inserted gene of human leukocyte interferon alpha-2, said strain being deposited in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1742; said strain being cultivated in a nutrient medium, containing the sources of carbon and nitrogen, mineral salts and growth stimulants, under aeration in the presence of antibiotics, i.e., streptomycin or tetracycline, or a mixture of both, followed by isolation and purification of the end product.

486749

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