Method for producing hybridisation complexes whose stability...

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2258936

The invention discloses a method for producing a hybridisation complex whose stability is substantially independent of the base composition of two hybridised nucleic acid molecules, consisting in contacting a first nucleic acid molecule with a second nucleic acid molecule under conditions enabling the formation of a specific hybridisation complex. The invention is characterised in that at least one of the four types of bases contained in the sequence of the first and/or of the second nucleic acid molecule is a modified base having pairing properties close to those of the natural base it replaces, such that at least one of the two types of the base pair contained in the composition of the complex comprises a modified base and in that the totality of the base pairs contained in the composition of the said complex has a like stability.

La présente invention concerne un procédé de formation d'un complexe d'hybridation dont la stabilité dépend peu de la composition en base des deux molécules d'acide nucléique hybridées, consistant à mettre en contact une première molécule d'acide nucléique avec une seconde molécule d'acide nucléique dans des conditions permettant la formation d'un complexe d'hybridation spécifique, caractérisé en ce que l'un au moins des quatre types de bases entrant dans la séquence de la première et/ou de la seconde molécule d'acide nucléique est une base modifiée possédant des propriétés d'appariement voisines de celles de la base naturelle qu'elle remplace, de façon à ce que l'un au moins des deux types de paire de bases entrant dans la composition du complexe comprenne une base modifiée et en ce que l'ensemble des paires de bases entrant dans la composition dudit complexe présente une stabilité voisine.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for producing hybridisation complexes whose stability... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing hybridisation complexes whose stability..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing hybridisation complexes whose stability... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-2010456

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.