Method for producing l-amino acid by fermentation

Details Method for producing l-amino acid by fermentation Method for producing l-amino acid by fermentation

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) C12N 15/52 (2006.01) C12N 15/70 (2006.01) C12P 13/06 (2006.01) C12P 13/08 (2006.01)

Patent

CA 2546678

L-threonine or L-isoleucine is produced by culturing a bacterium which belongs to the genus Escherichia and has an ability to produce L-threonine or L- isoleucine, and has a threonine operon of which expression is directed by its native promoter and from which at least a leader sequence and an attenuator are removed so that the attenuation is released, in a medium and collecting the L-threonine or L-isoleucine from the medium.

On produit de la thréonine L ou de l'isoleucine L par la culture d'une bactérie qui appartient au genre Escherichia et qui possède la capacité de produire de la thréonine L ou de l'isoleucine L et possède un opéron de thréonine dont l'expression est dirigée par son promoteur natif à partir duquel au moins une séquence leader et un atténuateur sont retirés de sorte que l'atténuation est libérée, cette production est obtenue dans un milieu et le recueil de la thréonine L ou de l'isoleucine L est effectué à partir de ce milieu.

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