Method for producing mature vwf from vwf pro-peptide

Details Method for producing mature vwf from vwf pro-peptide Method for producing mature vwf from vwf pro-peptide

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01) A61K 38/36 (2006.01) C07K 1/18 (2006.01) C07K 14/745 (2006.01)

Patent

CA 2684151

The present invention relates to a method for producing a mature von Willebrand Factor (VWF) from von Willebrand Factor pro-peptide comprising the steps: - immobilizing VWF pro-peptide on an ion exchange resin, incubating the immobilized VWF pro-peptide with furin to obtain immobilized mature VWF, and - isolating mature VWF from the ion exchange resin by elution.

La présente invention concerne un procédé destiné à produire un facteur de von Willebrand (VWF) mature à partir d'un propeptide du facteur de von Willebrand, comprenant les étapes consistant à : - immobiliser un propeptide du VWF sur une résine échangeuse d'ions, incuber le propeptide du VWF immobilisé avec de la furine afin d'obtenir un VWF mature immobilisé et - isoler le VWF mature de la résine échangeuse d'ions par élution.

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