Method for producing microbial cells and use thereof to...

C - Chemistry – Metallurgy – 12 – N

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195/33.1, 195/34

C12N 15/00 (2006.01) C07D 301/02 (2006.01) C07D 303/04 (2006.01) C12N 1/00 (2006.01) C12N 1/30 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/04 (2006.01) C12P 7/02 (2006.01) C12P 7/04 (2006.01) C12P 7/06 (2006.01) C12P 7/26 (2006.01) C12P 7/28 (2006.01) C12P 17/02 (2006.01)

Patent

CA 1143679

ABSTRACT OF THE DISCLOSURE Disclosed are newly discovered and isolated methylotrophic microorganism strains and their natural and/or artificial mutants which grow well under aerobic con- ditions in a culture medium in the presence of methane as the major carbon and energy source. The methane-grown microbial cells possess a high content of protein ane can be utilized as such as foodstuffs. The methane_-grown micro- bial cells or enzyme preparations thereof are also useful in converting C1-C6 alkanes to alcohols, particularly methane to methanol, C3-C6 alkanes to the corresponding C3-C6 sec. alcohols,methyl ketones, C3-C6 sec. alcohols to the corresponding methyl ketones, cyclic hydrocarbons to cyclic hydrocarbyl Alcohols (e.g., cyclohexane to cyclohexnnol) C2-C4 alkenes selected from the group consisting of ethylene, propylene, butene-1 and butadiene to 1-2-epoxides, styrene to styrene oxide, and converting other oxidizable substrates to oxidized products. The newly discovered and isolated methylotrophic microorgan- ism strains may be aerobically grown on n plurality of methyl-radical donating carbon-containing compounds, such as methanol, methylamine, methyl formate, methyl carbonate, dimethyl ether, etc. to produce microbial cells or enzyme preparations capable of aerobically converting C3-C6 linear secondary alcohols to the corresponding methyl ketones.

325437

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