Method for producing neural cell

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/079 (2010.01) C12N 5/0793 (2010.01) A61K 35/30 (2006.01) A61P 25/00 (2006.01) C12N 5/0735 (2010.01)

Patent

CA 2489206

It is intended to supply a large amount of nerve cells having been substantially isolated to thereby provide means applicable to nerve regeneration therapy for neurodegenerative diseases, nerve injury and so on. A process for producing nerve cells having been substantially isolated which comprises suspended-culturing embryonic stem cells in an acclimation medium for astrocytes or in the presence of components substantially equivalent to the acclimation medium; nerve cells thus obtained; a cell medicinal composition comprising the nerve cells as the active ingredient; and a method of treating a neurodegenerative disease or nerve injury by inserting the nerve cells into a neurodegenerative site or a nerve injury site.

L'invention permet d'obtenir une grande quantité de cellules nerveuses ayant été sensiblement isolées pour fournir un moyen applicable à une thérapie de régénération nerveuse, pour des maladies neurodégénératives, notamment des lésions nerveuses etc. L'invention concerne un procédé permettant de produire des cellules nerveuses sensiblement isolées comprenant des cellules souches embryonnaires de culture suspendues dans un milieu d'acclimatation, pour des astrocytes, ou en présence de composants sensiblement équivalents au milieu d'acclimatation. L'invention concerne les cellules nerveuses ainsi obtenues. L'invention concerne également une composition médicinale cellulaire comprenant les cellules nerveuses, en tant que principe actif, ainsi qu'un procédé de traitement d'une maladie neurodégénérative ou d'une lésion nerveuse, par insertion de ces cellules nerveuses dans un site neurodégénératif ou dans un site de lésion nerveuse.

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