Method for producing nucleoside-5'-phosphate ester

C - Chemistry – Metallurgy – 12 – N

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C12N 15/55 (2006.01) C12N 9/16 (2006.01) C12P 19/32 (2006.01)

Patent

CA 2221774

A process for efficiently and economically producing a nucleoside-5'-phosphate which comprises treating a nucleoside and a phosphoric acid donor selected from the group consisting of poly-phosphoric acid (salts), phenylphosphoric acid (salts) and carbamylphosphoric acid (salts) with an acid phosphatase, in particular one suffering from a decrease in the nucleotidase activity, at a pH value of 3.0 to 5.5 to thereby form a nucleoside-5'-phosphate and taking up the same.

Procédé permettant de produire de manière efficace et économique un nucléoside-5'-phosphate, consistant à traiter un nucléoside et un donneur d'acide phosphorique choisi dans le groupe constitué par un acide polyphosphorique (sels), un acide phénylphosphorique (sels) et un acide carbamylphosphorique (sels) avec une phosphatase acide, en particulier avec une phosphatase présentant une diminution de l'activité de nucléotidase, à une valeur de pH comprise entre 3 et 5,5 pour former un nucléoside-5'-phosphate, et à reprendre celui-ci.

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