Method for producing oligosaccharides

C - Chemistry – Metallurgy – 12 – P

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C12P 19/00 (2006.01) C12P 19/14 (2006.01)

Patent

CA 1334394

The present invention provides a method of producing oligosaccharides by the reaction between lactose and .beta.-galactosidase from Aspergillus oryzae in which an concentration of lactose in an enzyme reaction mixture is between 50 and 90% (w/v) and a reaction temperature is within the range of 55°C to the temperature at which the .beta.-galactosidase in the reaction mixture is inactivated. This invention therefore makes it possible to obtain the sugar mixture containing high-purity oligosaccharides, small amounts of unreacted lactose and by produced monosaccharides.

549981

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