Method for producing plants having an increased tolerance...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01)

Patent

CA 2328131

The invention relates to the use of nucleic acids which code a (poly)peptide with an intrinsic affinity to plasmodesmata, to the production of plants or parts thereof having an increased tolerance against drought and/or fungal infections and/or increased salt concentrations and/or extreme temperature (heat, cold), and to corresponding methods. According to the invention, a plant, a plant tissue or a plant cell is advantageously transfected with the nucleic acid. The nucleic acid preferably codes a virus-coded transport protein which, in an especially preferred embodiment, is a derivative of the pr17 protein with a hydrophilic N-terminal extension.

L'invention concerne l'utilisation d'acides nucléiques, lesquels codent pour un (poly)peptide avec une affinité intrinsèque pour des plasmodesmes, en vue de la production de végétaux ou de parties de ceux-ci présentant une tolérance élevée à la sécheresse et/ou aux infections fongiques et/ou aux concentrations salines élevées et/ou aux températures extrêmes (chaleur, froid), ainsi que des procédés correspondants. Avantageusement, on transfecte à cet effet un végétal, un tissu végétal ou une cellule végétale avec l'acide nucléique. De préférence, l'acide nucléique code pour une protéine de transport codée par un virus, laquelle est, dans un mode de réalisation particulièrement préféré, un dérivé de la protéine pr17 avec un prolongement N-terminal hydrophile.

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