C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/04 (2006.01) C12N 9/10 (2006.01) C12N 9/16 (2006.01) C12N 15/52 (2006.01) C12N 15/54 (2006.01) C12N 15/55 (2006.01) C12N 15/82 (2006.01) C12P 7/42 (2006.01)
Patent
CA 2365276
The present biotechnological approach for the production of polyhydroxyalkanoates (PHAs) uses microbial systems. The high production costs make them substantially more expensive than synthetic plastics. Engineering a novel pathway in eucaryotic cell systems is a beneficial alternative to the production of PHAs in bacteria. This pathway will initially produce free (C8) fatty acids from the fatty acid synthetic pathway through the action of thioesterase, that will then add a CoA moiety to the free fatty acid through the action of an acyl-CoA synthetase, that will produce 3-ketoacyl-CoAs from the acyl-CoA through the action of a thiolase, that will produce R-(-)-OH-acyl-CoAs from the 3-keto acid CoAs through the action of a dehydrogenase isoform from yeasts. These R-(-)-3-OH-acyl-CoAs will finally be used as substrate for the PHA synthase reaction.
L'approche biotechnologique de la présente invention qui permet de produire des polyhydroxyalcanoates (PHA) utilise des systèmes microbiens. Les coûts de production élevés rendent ces derniers sensiblement plus coûteux que les plastiques synthétiques. La mise au point d'un nouveau mécanisme dans des systèmes cellulaires eucaryotes est une solution de remplacement intéressante à la production de PHA dans des bactéries. Ce mécanisme produit au départ des acides gras libres (C8) à partir du mécanisme de synthèse des acides gras par l'action de la thioestérase, ceci ajoutant ensuite une fraction CoA à l'acide gras sous l'effet d'une synthétase acyl-CoA, ce qui produit du 3--cétoacyl-CoAs à partir de l'acyl-CoA sous l'effet d'une thiolase, ce qui produit du R-(-)-OH-acyl-CoAs à partir du 3-cétoacide CoAs sous l'effet d'une isoforme déshydrogénase provenant de levures. Ces R-(-)-3-OH-acyl-CoAs sont ensuite utilisés en tant que substrats pour la réaction de synthétase de PHA.
Aquin Stephanie
Vezina Louis-P.
Gowling Lafleur Henderson Llp
Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food
Universite Laval
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