Method for producing polyunsaturated c20 and c22 fatty acids...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2600286

The invention relates to a method for producing polyunsaturated fatty acids in transgenic plants, whereby nucleic acids coding for polypeptides with .DELTA.- 6-desaturase, .DELTA.-6-elongase, .DELTA.-5-desaturase, .DELTA.-5-elongase, .DELTA.-4-desaturase, .DELTA.-12-desaturase and/or .omega.-3-desaturase activity are introduced into the plants. Advantageously, said desaturases and elongases come from phytophtora sojae. The invention also relates to nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the inventive nucleic acid sequences, vectors containing the nucleic acid sequences and/or the nucleic acid constructs, and transgenic plants containing the cited nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to fatty acid compositions produced according to the inventive method, and to the use thereof.

La présente invention concerne un procédé de production d'acides gras polyinsaturés dans des plantes transgéniques, lequel procédé consiste à introduire dans des plantes des acides nucléiques codant pour des polypeptides présentant l'activité de la ?-6-désaturase, de la ?-6-élongase, de la ?-5-désaturase, de la ?-5-élongase, de la ?-4-désaturase, de la ?-12-désaturase et/ou de l'?-3-désaturase. Avantageusement, ces désaturases et élongases proviennent du soja Phytophtora sojae. L'invention concerne également les séquences d'acides nucléiques, des constructions d'acides nucléiques, vecteurs et organismes contenant les séquences d'acides nucléiques selon l'invention, des vecteurs contenant les séquences d'acides nucléiques et/ou les constructions d'acides nucléiques ainsi que des plantes transgéniques contenant les séquences d'acides nucléiques, constructions d'acides nucléiques et/ou vecteurs susmentionnés. Une autre partie de cette invention concerne des compositions d'acides gras produites par le procédé selon l'invention et leur utilisation.

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