Method for producing polyunsaturated fatty acids in...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2590329

The invention relates to a method for producing polyunsaturated fatty acids in an organism by introducing into the organism nucleic acids which encode polypeptides having .DELTA.-5-elongase, .DELTA.-6-desaturase, .DELTA.-5- desaturase, .DELTA.-4-desaturase, .DELTA.-12-desaturase and/or .DELTA.-6- elongase activity. Preferably, these desaturases and elongases are derived from Ostreococcus. The invention also relates to a method for producing oils and/or triacylglycerides having an increased content in long-chain polyunsaturated fatty acids. The invention further relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising the inventive nucleic acid sequences, to vectors comprising the nucleic acid sequences and/or to the nucleic acid constructs and to transgenic organisms comprising the aforementioned nucleic acid sequences, nucleic acid constructs and/or vectors. Another part of the invention relates to oils, lipids and/or fatty acids produced according to the inventive method and to the use thereof. The invention finally relates to fatty acids and triglycerides having an increased content in unsaturated fatty acids and to the use thereof.

Procédé de production d'acides gras polyinsaturés dans un organisme, qui consiste à introduire dans ledit organisme des acides nucléiques qui codent pour des polypeptides à activité .DELTA.-5-élongase, .DELTA.-6-désaturase, .DELTA.-5-désaturase, .DELTA.-4-désaturase, .DELTA.-12-désaturase et / ou .DELTA.-6-élongase. Ces désaturases et élongases proviennent de préférence d'Ostreococcus. La présente invention concerne en outre un procédé de production d'huiles et / ou de triacylglycérides ayant une forte teneur en acides gras polyinsaturés à chaîne longue. La présente invention concerne encore les séquences d'acide nucléique, les produits de recombinaison d'acide nucléique, les vecteurs et les organismes contenant les séquences d'acide nucléique selon la présente invention, des vecteurs contenant les séquences d'acide nucléique et / ou les produits de recombinaison d'acide nucléique ainsi que des organismes transgéniques contenant les séquences d'acide nucléique, les produits de recombinaison d'acide nucléique et / ou les vecteurs susmentionnés. Une autre partie de la présente invention concerne des huiles, des lipides et / ou des acides gras produits à partir du procédé selon la présente invention et leur utilisation. La présente invention concerne enfin des acides gras insaturés ainsi que des triglycérides ayant une forte teneur en acides gras insaturés et leur utilisation.

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