Method for producing polyunsaturated long-chain fatty acids...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) A01K 67/027 (2006.01) A23D 9/00 (2006.01) A23K 1/16 (2006.01) A23L 1/30 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/63 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2591599

The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, said nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.

L'invention concerne un procédé de production d'acides gras multi-insaturés dans un organisme, procédé consistant à introduire dans l'organisme, des acides nucléiques codant pour un polypeptide à activité acyl-CoA : lysophospholipidacyltransférase. Avantageusement, ces séquences d'acides nucléiques peuvent être exprimées dans l'organisme transgénique, éventuellement, avec d'autres séquences d'acides nucléiques codant pour un polypeptide du métabolisme acide gras ou lipidique. L'invention concerne en outre les séquences d'acides nucléiques conformes à l'invention, des constructions d'acides nucléiques renfermant lesdites séquences d'acides nucléiques selon l'invention, des vecteurs renfermant les séquences d'acides nucléiques selon l'invention et/ou les constructions d'acides nucléiques, ainsi que les organismes transgéniques renfermant les séquences d'acides nucléiques précitées, les constructions d'acides nucléiques et/ou les vecteurs précités. L'invention concerne en outre des huiles, des lipides et/ou des acides gras fabriqués conformément au procédé de l'invention, ainsi que l'utilisation de ces composés.

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