Method for producing retinal nerve cells from neural...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/079 (2010.01) A61K 35/30 (2006.01) A61K 35/44 (2006.01)

Patent

CA 2528426

A process for producing retinal neurocytes, comprising conducting differentiation induction of iris pigmented epithelial cells into retinal neurocytes. The first process comprises co-culturing iris pigmented epithelial cells derived from a mammal and embryo retinal stem cells derived from a bird. The second process comprises isolating iris pigmented epithelial cells of a bird, a mammal, etc. and subjecting the iris pigmented epithelial cells to stationary culture. In these processes, retinal neurocytes can be produced with the use of iris pigmented epithelial cells collected from a patient per se, so that realization of highly effective regenerative medicine is promising.

L'invention concerne un procédé permettant de produire des neurocytes rétiniens, consistant à induire la différenciation des cellules épithéliales pigmentées de l'iris en neurocytes rétiniens. Un premier procédé consiste à mettre en coculture des cellules épithéliales pigmentées d'iris dérivées d'un mammifère, et des cellules souches rétiniennes embryonnaires obtenues à partir d'un oiseau. Un second procédé consiste à isoler les cellules épithéliales pigmentées de l'iris d'un oiseau, d'un mammifère etc., et à mettre ces cellules épithéliales pigmentées d'iris en culture stationnaire. Ces procédés permettent de produire des neurocytes rétiniens en utilisant les cellule épithéliales pigmentées de l'iris prélevées sur le patient lui-même, offrant ainsi des perspectives prometteuses pour des traitements régénératifs hautement efficaces.

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