Method for producing scyllo-inositol

Details Method for producing scyllo-inositol Method for producing scyllo-inositol

C - Chemistry, Metallurgy

12

P

C12P 7/18 (2006.01) C07C 35/16 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 15/53 (2006.01) C12P 7/26 (2006.01)

Patent

CA 2542560

It is intended to provide a novel NAD+-independent myo-inositol 2- dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacteror Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Further, scyllo- inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

L'invention concerne une nouvelle myo-inositol 2-déshydrogénase NAD?+¿-indépendante permettant de convertir le myo-inositol en scyllo-inosose en l'absence de NAD?+ ¿; une nouvelle enzyme, la scyllo-inositol déshydrogénase, qui réduit de façon stéréospécifique le scyllo-inosose en scyllo-inositol en présence de NADH ou de NADPH ; et un nouveau microorganisme appartenant au genre Acetobacter ou Burkholderia et qui permet de convertir le myo-inositol en scyllo-inositol. L'utilisation de ces enzymes ou de ce microorganisme permet de produire du scyllo-inositol. De plus, le scyllo-inositol est purifié par adjonction d'acide borique et d'un sel métallique à un mélange liquide contenant du scyllo-inositol et un saccharide neutre autre que le scyllo-inositol pour former un complexe scyllo-inositol/acide borique, par séparation du complexe à partir du mélange liquide, par dissolution du complexe ainsi séparé dans un acide pour obtenir une solution acide ou une suspension acide, puis par purification du scyllo-inositol à partir de la solution acide ou de la suspension acide.

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