Method for producing the hincii restriction endonuclease and...

Details Method for producing the hincii restriction endonuclease and... Method for producing the hincii restriction endonuclease and...

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 15/55 (2006.01) C12N 9/10 (2006.01) C12N 9/16 (2006.01) C12N 9/22 (2006.01)

Patent

CA 2012149

The present invention is directred to a method for cloning and producing the HincII restriction endonuclease by 1) introducing the restriction endonuclease gene from Haemophilus influenzae Rc into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing HincII restriction endonuclease, and 3) purifying the HincII restriction endonuclease from the fermented host which contains the vector encoding and expressing the HincII restriction endonuclease activity.

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