Method for producing tissue cells from pluripotent stem...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/071 (2010.01) C12N 5/077 (2010.01) A61K 35/44 (2006.01)

Patent

CA 2528870

A method for producing tissue cells derived from iris pigmented epithelial cells of an animal, and tissue cells obtained by the method are provided. The method and the tissue cells solve problems such as immunological rejection in cell transplantation, ethical issues, and unbalance between the demand and supply of transplant cell sources. In the method of the present invention for producing the tissue cells, first, the iris pigmented epithelial cells isolated from an eyeball of an animal are selectively cultured according to a floated coagulated mass culturing technique so as to obtain pluripotent stem cells. Thereafter, the pluripotent stem cells are cultured by using, for example, serum so as to produce various tissue cells.

La présente invention concerne un procédé de production de cellules tissulaires dérivant de cellules épithéliales du pigment de l'iris d'un animal. Ce procédé, qui évite le rejet immunologique découlant de la transplantation de cellules, et apporte une réponse élégante aux questions d'éthique, permet de s'affranchir du déséquilibre entre l'offre et la demande pour les sources de cellules transplantables. En outre, le procédé permet la production de cellules tissulaires. En l'occurrence, pour ce procédé de production de cellules tissulaires, on commence par cultiver sélectivement des cellules épithéliales du pigment de l'iris prélevées dans des globes oculaires d'animaux. On procède par culture en masse coagulée flottante jusqu'à obtention de cellules souches pluripotentes. Ces cellules souches pluripotentes subissent ensuite une culture au sérum de façon à produire diverses cellules tissulaires.

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