Method for producing tooth

A - Human Necessities – 61 – L

Patent

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Details

A61L 27/00 (2006.01) C12N 5/077 (2010.01)

Patent

CA 2750072

A method for designing a method for producing a single tooth under a predetermined condition, wherein the designing method includes a method for determining, when the first cell aggregate and the second cell aggregate are arranged in close contact inside a support carrier and the first cell aggregate and the second cell aggregate are respectively composed of either one of mesenchymal cells and epithelial cells, a maximum contact length of the both cell aggregates, which is required for producing a single tooth, and wherein the method for determining the maximum contact length further includes: a step of producing a plurality of types of structures in which the first cell aggregate and the second cell aggregate are arranged in close contact inside a support carrier by changing the maximum contact length of the first cell aggregate and the second cell aggregate, wherein the first cell aggregate and the second cell aggregate are respectively composed of either one of mesenchymal cells and epithelial cells; a step of culturing each of the plurality of types of structures inside the support carrier; and a step of measuring the number of teeth produced in the preceding step and determining the maximum contact length of the first cell aggregate and the second cell aggregate which is required for obtaining a single tooth.

La présente invention concerne un procédé de production d'une dent ayant une longueur souhaitée dans un sens. Spécifiquement, l'invention concerne un procédé de production d'une dent ayant une longueur souhaitée dans un sens comprenant les étapes consistant à : placer un premier agrégat de cellules et un second agrégat de cellules à l'intérieur d'un support tout en rapprochant étroitement les premier et second agrégats de cellules l'un de l'autre ; et cultiver les premier et second agrégats de cellules à l'intérieur du support, le premier agrégat de cellules étant composé de cellules mésenchymateuses ou de cellules épithéliales et le second agrégat de cellules étant composé des autres cellules, et la taille de la dent étant contrôlée en ajustant la longueur de contact entre le premier agrégat de cellules et le second agrégat de cellules dans un sens.

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