Method for producing yeast expressed hpv types 6 and 16...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/37 (2006.01) A61K 39/12 (2006.01) C07K 14/205 (2006.01) C12N 7/04 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2340422

Mosaic VLPs of viral capsid proteins from different virus types are described, as are methods of making the same. Specifically, a diploid yeast strain that coexpresses the L1 and L2 capsid proteins of both HPV-6 and HPV-16 as mosaic VLPs is described. The mosaic VLPs induced the production conformational antibodies against both L1 proteins upon administration to mice.

Cette invention décrit des particules vériformes (VLP) mosaïques de protéines de capsides virales provenant de différents types de virus, ainsi que des procédés de production de ces particules. Cette invention décrit spécifiquement une souche de levure diploïde qui exprime conjointement les protéines de capsides L1 et L2 des deux papillomavirus humains PVH-6 et PVH-16 en tant que particules vériformes (VLP) mosaïques. Ces particules vériformes mosaïques ont induit la production d'anticorps conformationnels contre les deux protéines L1, lors de leur administration à des souris.

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