Method for production of a compound in a eukaryotic cell

Details Method for production of a compound in a eukaryotic cell Method for production of a compound in a eukaryotic cell

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/38 (2006.01) C07K 14/705 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2580884

The present invention relates to a eukaryotic cell containing peroxisomes that are capable to fuse with a membrane-structure of the cell involved in the secretory pathway of the cell. In this way, the eukaryotic cell is able to release the peroxisomal content outside the cell. The invention also relates to a method for production of a compound of interest in said eukaryotic cell wherein said compound of interest is present in the peroxisome of the cell. Said compound of interest will accumulate in the peroxisome by a signal promoting peroxisome localisation. Preferred host cells are filamentous fungal cells.

La présente invention a trait à une cellule eucaryote contenant des peroxysomes qui sont capables de fusionner avec une structure membranaire de la cellule impliquée dans la voie sécrétoire de la cellule. Ainsi, la cellule eucaryote est capable de libérer le contenu peroxysomal hors de la cellule. L'invention a également trait à un procédé pour la production d'un composé d'intérêt dans ladite cellule eucaryote, ledit composé d'intérêt étant présent dans le peroxysome de la cellule. Ledit composé d'intérêt va s'accumuler dans le peroxysome grâce à un signal favorisant la localisation de peroxysomes. Des cellules hôtes préférées sont des cellules de champignons filamenteux.

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