Method for production of asymmetric carotenoids

Details Method for production of asymmetric carotenoids Method for production of asymmetric carotenoids

C - Chemistry, Metallurgy

12

P

C12P 1/02 (2006.01) C07H 21/04 (2006.01) C12N 9/02 (2006.01) C12N 9/88 (2006.01) C12P 1/04 (2006.01) C12P 1/06 (2006.01) C12P 7/22 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2468096

Genes have been isolated from Rhodococcus and Deinococcus which encode a specific lycopene .beta.-cyclase capable of converting acyclic carotenoids with at least one .psi.-end group to the corresponding asymmetric carotenoid containing a single .beta.-ionone ring end group. The genes are new. Transformed host cells expressing the present genes and methods for the bio- conversion of acylic carotenoid substrates to corresponding asymmetric carotenoid are also provided.

L'invention concerne des gènes ayant été isolés à partir de <i>Rhodococcus </i>et de <i>Deinococcus</i>, codant un lycopène .beta.-cyclase spécifique, pouvant convertir des caroténoïdes acycliques présentant au moins un groupe d'extrémité .psi. en caroténoïde asymétrique correspondant contenant un groupe d'extrémité d'anneau de .beta.-ionone unique. Ces gènes sont de nouveaux gènes. L'invention concerne également des cellules hôtes transformées exprimant les gènes susmentionnés et des procédés pour la bioconversion de substrats de caroténoïdes acycliques en caroténoïde asymétrique correspondant.

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