Method for production of human tissue type plasminogen...

Details Method for production of human tissue type plasminogen... Method for production of human tissue type plasminogen...

C - Chemistry, Metallurgy

12

N

195/127, 195/72

C12N 9/64 (2006.01) C12N 5/00 (2006.01) C12N 9/72 (2006.01)

Patent

CA 1335188

Human tissue type plasminogen activator (tPA) which is effective as a therapeutic agent is obtained at a high concentration by increasing the osmotic pressure of the cell culture medium or tPA producing medium using bicarbonate ion up to at least 350 milliosmole/liter optionally also supplementing the medium with an antiplasmin agent. Single- chain tPA is produced at a high concentration accordingly.

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