Method for production of native human leukocyte (alpha)...

Details Method for production of native human leukocyte (alpha)... Method for production of native human leukocyte (alpha)...

C - Chemistry, Metallurgy

07

K

C07K 14/56 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2234396

The invention relates to a process for the preparation of natural human alfa interferon from human blood by ammonium chloride haemolysis and purification by washing with buffered physiological saline solution, then preparing a suspension in a liquid culture medium and induction by Sendai virus. The process according to the invention is characterized in that leukocytes are separated from human blood, purified in a known way, suspended in a liquid culture medium, then induced by Sendai virus, and the pre-treatment is carried out at 30-40°C, 60-90 minutes after the induction the incubation is continued at 30°C or at a lower temperature, at the end of incubation further quantity of Sendai virus is added to the suspension, then the cells are separated, and the pH of the supernatant is made acidic.

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