Method for production of neurons from cells of a cell line

Details Method for production of neurons from cells of a cell line Method for production of neurons from cells of a cell line

C - Chemistry, Metallurgy

12

N

C12N 5/0793 (2010.01) A61K 35/30 (2006.01) C12N 11/00 (2006.01)

Patent

CA 2536972

The invention relates to a method for production of neurons from cells of a cell line which may be differentiated to produce neurons in particular, whereby said cells are cultivated in spheres, preferably by exposing the same to growth factors, such as, for example, EGF (epidermal growth factor) and/or bFGF (basic fibroblast growth factor) or LIF (Leukemia Inhibitory Factor), in a given growth medium, the differentiation in said spheres is induced on forcing the same to adhere to a substrate, after removal of the growth factors EGF and/or bFGF or LIF, and cultivating the same in the growth medium for an appropriate duration. Said method is characterised in that cells of the human embryonic teratocarcinoma NT2 are used.

L'invention a trait à un procédé de production de neurones à partir de cellules d'une lignée cellulaire apte à se différencier pour produire notamment des neurones, dans lequel : - on cultive lesdites cellules en sphères, de préférence en les exposant à des facteurs de croissance, Lels que, par exemple, EGF (epidermal growth factor) et/ou bFGF (basic fibroblast growth factor) ou du LIF (Leukemia Inhibitory Factor), dans un milieu de croissance défini, - on induit la différenciation desdites sphères en les faisant adhérer sur un substrat, après élimination des facteurs de croissance EGF et/ou bFGF ou LIF, et en les cultivant dans le milieu de croissance pendant un laps de temps approprié. Ce procédé est caractérisé en ce que l'on utilise les cellules de la lignée de tératocarcinome embryonnaire humain NT2.

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