Method for production of plant cell having chromosome loss

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) A01H 5/00 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2590076

Disclosed is a method for readily producing an aneuploid, which is applicable to any species and does not cause any unexpected damage to chromosomes other than a chromosome to be lost. The method comprises steps of: introducing a set of inverted recognition sequences running in opposite directions or a single recognition sequence comprising a nucleotide sequence which is symmetrical about a given point together with a vector carrying a gene for a recombinant enzyme into plant cells or, alternatively, introducing a vector carrying a set of inverted recognition sequences running in opposite directions or a single recognition sequence comprising a nucleotide sequence which is symmetrical about a given point into plant cells and then allowing to act a recombinant enzyme on the cells transiently at least during the growth of the cells; culturing the cells to grow; and selecting cells in which a predetermined chromosome has been lost.

La présente invention a trait à un procédé pour la production simple et facile d'un aneuploïde, qui est applicable à n'importe quelle espèce et n'entraîne aucun dommage imprévu aux chromosomes autres qu'un chromosome à éliminer. Le procédé comprend les étapes suivantes: l'introduction d'un ensemble de séquences de reconnaissance inversées s'étendant dans des directions opposées ou une unique séquence de reconnaissance inversée comportant une séquence de nucléotides qui est symétrique autour d'un point conjointement avec un vecteur portant un gène pour une enzyme recombinante dans des cellules végétales ou, en variante, l'introduction d'un vecteur portant un ensemble de séquences de reconnaissance inversées comportant une séquence de nucléotides qui est symétrique autour d'un point dans des cellules végétales en permettant l'action transitoire d'une enzyme recombinante sur les cellules au moins pendant la croissance des cellules; la culture des cellules en vue de leur croissance; et la sélection de cellules dans lesquelles un chromosome prédéterminé a été éliminé.

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