Method for promoting fusion of plant protoplast

Details Method for promoting fusion of plant protoplast Method for promoting fusion of plant protoplast

C - Chemistry, Metallurgy

12

N

195/1.101

C12N 5/02 (2006.01) C12N 5/14 (2006.01) C12N 15/00 (2006.01)

Patent

CA 1220733

- 24 - METHOD FOR PROMOTING FUSION OF PLANT PROTOPLAST ABSTRACT OF THE DISCLOSURE A method for promoting fusion of plant protoplasts comprising the steps of; (a) treating the plant proto- plasts with a fusing agent, and then (b) inclubating the resultant plant protoplasts in an incubation medium having an osmotic pressure lower than that required for maintaining the plant protoplasts in a stable form. This effectively promotes the fusion of the plant proto- plasts at a higher fusion rate in a wider range of plants.

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