Method for protein binding enzyme complementation assays

Details Method for protein binding enzyme complementation assays Method for protein binding enzyme complementation assays

C - Chemistry, Metallurgy

12

N

195/1.22, 195/1.

C12N 15/62 (2006.01) C07K 14/02 (2006.01) C07K 14/59 (2006.01) C12N 9/38 (2006.01) C12N 9/96 (2006.01) C12N 15/56 (2006.01) G01N 33/535 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1340328

This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.

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