Method for protein binding enzyme complementation assays

Details Method for protein binding enzyme complementation assays Method for protein binding enzyme complementation assays

C - Chemistry, Metallurgy

12

N

167/37, 150/15.2

C12N 15/56 (2006.01) C12N 1/21 (2006.01) C12N 9/38 (2006.01) G01N 33/535 (2006.01) G01N 33/542 (2006.01) G01N 33/557 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2025929

This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are describe.

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