Method for purification of borrelia lipoprotein ospa

Details Method for purification of borrelia lipoprotein ospa Method for purification of borrelia lipoprotein ospa

C - Chemistry, Metallurgy

07

K

C07K 14/20 (2006.01) C07K 1/36 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2333167

The present invention provides a method for purification of Borrelia lipoprotein OspA suitable for human clinical use whereby a host cell expressing lipoprotein OspA is lysed in the presence of a zwitterionic detergent followed by several chromatographic steps in a nonionic detergent.

La présente invention concerne un procédé de purification de la lipoprotéine OspA Borrelia approprié pour une utilisation clinique chez l'homme. Dans ledit procédé, une cellule hôte exprimant la lipoprotéine OspA est lysée en présence d'un détergent zwittérionique, puis soumise à un processus chromatographique à plusieurs phases dans un détergent non ionique.

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