Method for purification of filamentous hemagglutinin

Details Method for purification of filamentous hemagglutinin Method for purification of filamentous hemagglutinin

C - Chemistry, Metallurgy

12

P

167/135, 195/78,

C12P 21/00 (2006.01) A61K 39/10 (2006.01) C07K 14/235 (2006.01) A61K 39/00 (2006.01)

Patent

CA 1237998

- 1 - Abstract: The invention provides an improved method for the purification of filamentous hemagglutinin (F-HA) on an industrial scale which comprises contacting a culture of a microorganism of the genus Bordetella with a cellulose sulfate gel, a polysaccharide gel chemically bound with dextran sulfate, or a crosslinked polysaccharide sulfate gel, thereby adsorbing the F-HA on the gel, and then eluting the F-HA from the gel. The method can give a highly purified F-HA which does not contain any other proteins, lipids, saccharides, etc. and undesirable endotoxins, and hence can be used for producing various reagents, medicines and pertussis vaccine.

479022

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