Method for purification of hbs antigen

A - Human Necessities – 61 – K

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A61K 39/12 (2006.01)

Patent

CA 1108051

METHOD FOR PURIFICATION OF HBs ANTIGEN Abstract of the Disclosure The specification discloses an improved method for the purification of HBS antigen comprising passing a partially purified HBs antigen prepared from blood plasma or serum through a column filled with an anion exchanger which may be equilibrated with a buffer solution having a specific ionic strength and pH level, and further passing the resulting effluent through a column filled with a cation exchanger which may be equilibrated with a buffer solution having a specific ionic strength and pH level. The HBs antigen obtained by the present invention is highly pur- ified and is useful for preparing vaccine for preventing Viral Hepatitis Type B.

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