Method for purification of nucleic acids, particularly from...

Details Method for purification of nucleic acids, particularly from... Method for purification of nucleic acids, particularly from...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2746489

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

L'invention porte sur un procédé de purification d'acides nucléiques, sur une trousse destinée à mettre en uvre le procédé selon l'invention, ainsi que sur une nouvelle utilisation de particules magnétiques pour purifier un échantillon biologique. Le procédé selon l'invention comprend les étapes suivantes : a) mise en place de l'échantillon dans un premier récipient porte-échantillon, en solution aqueuse, et lyse de l'échantillon dans des conditions non chaotropiques ; mise en suspension des premières particules magnétiques dans la solution, et mise en place du premier récipient porte-échantillon dans un rack de récipients porte-échantillon, le récipient porte-échantillon étant introduit dans l'espace intérieur annulaire d'un aimant annulaire associé au rack de récipients porte-échantillon ; séparation de la solution d'avec les particules magnétiques ; et isolement des acides nucléiques d'avec la solution.

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